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<div id="zrjhthtj" class="clearfix"></div> </div> <div id="zrjhthtj" class="HeightTab clearfix"></div> </div>  <div id="zrjhthtj" class="right"> <div id="zrjhthtj" class="Position"><span>你的位置：<a href="/">首頁</a> > <a href='/News/'>新聞動態(tài)</a> > <a href='/News/IndustryNews/'>行業(yè)新聞</a></span></div> <div id="zrjhthtj" class="HeightTab clearfix"></div>  <div id="zrjhthtj" class="main">  <div id="zrjhthtj" class="content"> <div id="zrjhthtj" class="title"><h3>色譜填料技術 —— 色譜柱整體效果的關鍵所在</h3></div> <div id="zrjhthtj" class="infos">2016-10-28 15:13:47??????點擊：<script language="javascript" src="/inc/article_view.asp?id=1600"></script></div> <div id="zrjhthtj" class="maincontent clearfix"> <div>色譜是一種分離分析手段，分離是核心，因此擔負分離作用的色譜柱是色譜系統(tǒng)的心臟，色譜填料則是色譜技術的核心。除了固定相性能外，色譜填料技術對色譜柱的整體效果也起著至關重要的作用，裝填技術是獲得高效液相色譜柱的重要環(huán)節(jié)。</div> <div><br /> </div> <div>　　填料</div> <div><br /> </div> <div>　　常見的分配柱填料包括：碳十八柱[1]（ODS/C18)、碳八柱（MOS/C8）、碳六柱（Hexyl/C6）、碳四柱（Butyl/C4）、碳一柱（Methyl/C1）、陰離子交換柱（SAX)、陽離子交換柱（SCX）、苯基柱（Phenyl）、氨基柱（Amino/NH2）、氰基柱（Cyano/CN/Nitrile）。常見的吸附柱填料有硅膠柱。隨著科技的快速發(fā)展，色譜柱填料也在不斷翻開新篇章。出現(xiàn)了石墨化碳填料、貫流色譜填料和整體柱。</div> <div><br /> </div> <div>　　整體柱是一種新型的用于分離分析或作為反應器的多孔介質。早在2010年，色譜行業(yè)專家于泓教授便認為整體柱將會成為一個發(fā)展趨勢。那時市面上已經(jīng)出現(xiàn)了一些硅膠型整體柱的產(chǎn)品，比如默克公司已生產(chǎn)出反相整體柱。同傳統(tǒng)色譜柱球形顆粒，填充緊密，間隙較小的特點不同，整體柱的空間由聚合物顆粒中的孔和顆粒間的縫隙組成。當流動相經(jīng)過大孔時，阻力小，使得液相色譜的流速提高，但柱壓不會明顯升高，高流速可以達到10ml/min(使用填充柱流速一般為1ml/min～1.5ml/min之間)。分離在樣品流經(jīng)孔結構時發(fā)生，可以減少路徑的差異和縱向擴展。所以整體柱具有快速分離，穩(wěn)定性很好的突出特點。</div> <div><br /> </div> <div>　　同填料一起發(fā)展的還有填料技術。利用亞2μm小粒徑硅膠、核殼型硅膠已成為填料技術發(fā)展一大趨勢。該技術擁有非常豐富的選擇性。包括HILIC色譜填料、極性嵌入反相色譜填料、立體保護鍵合相、有機-無機雜化色譜填料、親水性體積排阻(SEC)色譜填料、混合模式色譜填料、手性色譜填料和有機高分子基質液相色譜填料。2μm填料粒徑還可以實現(xiàn)高速、高分辨率、高靈敏度的分離分析。目前，市面上能生產(chǎn)2μm色譜柱的企業(yè)還不多。</div> <div><br /> </div> <div>　　近年來，我色譜技術得到飛速發(fā)展，相關產(chǎn)學研活動也蓬勃開展。隨著市場的進一步拓寬，色譜技術將為我們帶來更多更便利的應用。</div> <div><ul 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